• Direct imaging of IgE on the mica surface by tapping-mode atomic force microscopy

      Hu, Jing; Wang, Ying; Gao, Mingyan; Song, Zhengxun; Chen, Yujuan; Wang, Zuobin; Changchun University of Science and Technology; University of Bedfordshire (IEEE, 2020-02-02)
      Immunoglobulin E (IgE) antibody is essential in the functioning of the immune system, so the study of IgE has its practical and profound significance. Herein, the effect of protein concentration and adsorption time on IgE morphology of mica surface was investigated. For this purpose, atomic force microscopy (AFM) has been performed for monitoring protein morphology at different concentrations and adsorption times. In addition, the height and average roughness of IgE were also obtained. The changes of IgE molecule morphology including the shape, height and average roughness indicated that the interactions of protein-surface and protein-protein were varying with the protein concentration and adsorption time.
    • A disulfiram-loaded fibers scaffold fabricated via electrospinning method

      Xie, Chenchen; Ding, Ran; Wang, Xinyue; Yan, Jin; Wang, Ying; Zhang, Wenxiao; Qu, Yingmin; Wang, Zuobin; Changchun University of Science and Technology; University of Bedfordshire (IEEE, 2020-01-02)
      In this study, we developed a disulfiram-loaded fibers scaffold via the electrospinning method for enhancing the stability of disulfiram and facilitating the appropriate distribution in tumor tissues. The drug release profile of the disulfiram-loaded fibers scaffold was examined by high-performance liquid chromatography. The results showed that both the initial burst release and the subsequent sustainable release of the drug were suitable for cancer treatments. The results of an MTT assay, which tested the therapeutic efficacy of electrospun fibers in vitro, showed that the DSF-PVDF fibers exhibited their anticancer activity due to the incorporation of DSF. It indicates that DSF is successfully incorporated into the electrospun fibers and the resultant electrospun fibers are highly promising for cancer treatments.
    • Fabrication of TiO2 nanowire arrays using laser interference lithography aided hydrothermal method

      Ning, Xiao-Hui; Meng, Qing-Ling; Li, Li; Han, Yong-Lu; Zhou, Dong-Yang; Cao, Liang; Weng, Zhankun; Ding, Ran; Wang, Zuobin; Changchun University of Science and Technology; et al. (IEEE, 2018-02-08)
      Titanium dioxide (TÍO 2 ) is one of the most widely investigated semiconductor materials because of its unique properties. TiO 2 nanowire arrays can be synthesized through a two-step method, the fluorine-doped tin oxide (FTO) substrates were ablated using laser interference lithography technology, and TiO 2 nanowire arrays grown on the patterned FTO substrates. The TiO 2 nanowire arrays were characterized by SEM and XRD measurements. This work provides a high efficient method for the fabrication of ordered TiO 2 nanowire arrays for different applications in highly functionalized assemblies and composites.
    • Growth of nerve cells induced by diverse nanopillar arrays

      Liu, Mengnan; Dong, Litong; Yang, Xueying; Guo, Xuan; Wang, Xuan; Xie, Chenchen; Song, Zhengxun; Wang, Zuobin; Li, Dayou; Changchun University of Science and Technology; et al. (IEEE, 2020-01-02)
      The nanotopographies can induce the growth of nerve cells and the growth of their synapses. Studying the anisotropic structures for the guidance of neuronal synapses is beneficial to the in vitro repair of neurons and the development of regenerative medicine. Thus, studying how diverse nanopillar arrays affect the growth of nerve cells is essential. This paper employed the technology of laser interference lithography (LIL) to fabricate different nanopillar arrays with the same and different size gaps between the X and Y directions, and observe how the structures induce the growth of nerve cells and their synapses.
    • Imaging quality assessment of different AFM working modes on living cancer cells

      Wang, Guoliang; Sun, Baishun; Wu, Xiaomin; Zhang, Wenxiao; Qu, Yingmin; Song, Zhengxun; Wang, Zuobin; Li, Dayou; Changchun University of Science and Technology; University of Bedfordshire (IEEE, 2020-02-02)
      Since the invention of atomic force microscope (AFM) in 1986, its capabilities in biophysical research, such as living cell imaging, molecule imaging and recognition and drug treatment analysis, have been deeply investigated. Various types of working modes of atomic force microscopy have been employed for imaging and analyzing living cells. The physical properties of living cells can be directly illustrated by its good resolution images. In this paper, the applications of three AFM working modes including contact, tapping and quantitative imaging (QI) modes for the investigation of living lung cancer cells (A549) are presented. Meanwhile, the quality of images of the cells obtained by different working modes is compared through the image quality assessment (IQA) methods.