AFM-detected apoptosis of hepatocellular carcinoma cells induced by American ginseng root water extract
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Authors
Qu, YingminWang, Zuobin
Zhao, Feihu
Liu, Jinyun
Zhang, Wenxiao
Li, Jingmei
Song, Zhengxun
Xu, Hongmei
Affiliation
Changchun University of Science and TechnologyEindhoven University of Technology
University of Bedfordshire
Issue Date
2017-10-12Subjects
ginseng-extracted compoundsAmerican ginseng
atomic force microscope
atomic force microscopy
cell apoptosis
cellular stiffness
SMMC-7721 cells
hepatocelluar carcinoma
C130 Cell Biology
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Show full item recordAbstract
American ginseng as a common and traditional herbal medicine has been used in cancer treatment for many years. However, the effect of American ginseng on the cancer cell response (i.e. apoptosis) has not been fully understood yet. Previous studies demonstrated that cellular apoptosis was associated with the changes of mechanical and morphological properties. Therefore, in this study, mechanical and morphological characterizations were carried out by both atomic force microscope (AFM) and inverted optical microscope to investigate the apoptosis of hepatocellular carcinoma (SMMC-7721) cells affected by American ginseng root water extract (AGRWE). The results showed that the cells treated with AGRWE exhibited significantly larger surface roughness, height and elastic modulus values than control group. Moreover, those parameters were upregulated under the higher concentration of AGRWE and longer culture time. Consequently, it indicates that the mechanical and morphological properties can be used as the apoptotic characteristics of SMMC-7721 cells. Also, the increased surface roughness and elastic modulus of cells under the AGRWE treatment have shown that the apoptosis of SMMC-7721 cells can be enhanced by AGRWE. This will provide an important implication for hepatocelluar carcinoma treatment and drug development.Citation
Qu Y, Wang Z, Zhao F, Liu J, Zhang W, Li J, Song Z, Xu H (2018) 'AFM-detected apoptosis of hepatocellular carcinoma cells induced by American ginseng root water extract', Micron, 104, pp.1-7.Publisher
ElsevierJournal
MicronPubMed ID
29049926Additional Links
https://www.sciencedirect.com/science/article/pii/S0968432817303256Type
ArticleLanguage
enISSN
0968-4328Sponsors
“111” Project of China (D17017), EU FP7 (BioRA No.612641), China-EU H2020 (FabSurfWAR Nos.2016YFE0112100 and 644971), EU H2020 (MNR4SCell No.734174), and Jilin Provincial Science and Technology Program (Nos.20160520101JH, 20160101318JC and 20160623002TC)ae974a485f413a2113503eed53cd6c53
10.1016/j.micron.2017.10.003
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