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dc.contributor.authorGibson, Oliver R.
dc.contributor.authorTuttle, James A.
dc.contributor.authorWatt, Peter W.
dc.contributor.authorMaxwell, Neil S.
dc.contributor.authorTaylor, Lee
dc.contributor.illustrator
dc.date.accessioned2021-08-13T11:54:37Z
dc.date.available2021-08-13T00:00:00Z
dc.date.available2021-08-13T11:54:37Z
dc.date.issued2016-08-11
dc.identifier.citationGibson O, Tuttle J, Watt P, Maxwell N, Taylor L (2016) 'Hsp72 and Hsp90α mRNA transcription is characterised by large, sustained changes in core temperature during heat acclimation', Cell Stress and Chaperones, 21 (6), pp.1021-1035.en_US
dc.identifier.issn1355-8145
dc.identifier.pmid27511024
dc.identifier.doi10.1007/s12192-016-0726-0
dc.identifier.urihttp://hdl.handle.net/10547/625086
dc.description.abstractIncreased intracellular heat shock protein-72 (Hsp72) and heat shock protein-90α (Hsp90α) have been implicated as important components of acquired thermotolerance, providing cytoprotection during stress. This experiment determined the physiological responses characterising increases in Hsp72 and Hsp90α mRNA on the first and tenth day of 90-min heat acclimation (in 40.2 °C, 41.0 % relative humidity (RH)) or equivalent normothermic training (in 20 °C, 29 % RH). Pearson’s product-moment correlation and stepwise multiple regression were performed to determine relationships between physiological [e.g. (Trec, sweat rate (SR) and heart rate (HR)] and training variables (exercise duration, exercise intensity, work done), and the leukocyte Hsp72 and Hsp90α mRNA responses via reverse transcription quantitative polymerase chain reaction (RT-QPCR) (n = 15). Significant (p < 0.05) correlations existed between increased Hsp72 and Hsp90α mRNA (r = 0.879). Increased core temperature was the most important criteria for gene transcription with ΔTrec (r = 0.714), SR (r = 0.709), Trecfinal45 (r = 0.682), area under the curve where Trec ≥ 38.5 °C (AUC38.5 °C; r = 0.678), peak Trec (r = 0.661), duration Trec ≥ 38.5 °C (r = 0.650) and ΔHR (r = 0.511) each demonstrating a significant (p < 0.05) correlation with the increase in Hsp72 mRNA. The Trec AUC38.5 °C (r = 0.729), ΔTrec (r = 0.691), peak Trec (r = 0.680), Trecfinal45 (r = 0.678), SR (r = 0.660), duration Trec ≥ 38.5 °C (r = 0.629), the rate of change in Trec (r = 0.600) and ΔHR (r = 0.531) were the strongest correlate with the increase in Hsp90α mRNA. Multiple regression improved the model for Hsp90α mRNA only, when Trec AUC38.5 °C and SR were combined. Training variables showed insignificant (p > 0.05) weak (r < 0.300) relationships with Hsp72 and Hsp90α mRNA. Hsp72 and Hsp90α mRNA correlates were comparable on the first and tenth day. When transcription of the related Hsp72 and Hsp90α mRNA is important, protocols should rapidly induce large, prolonged changes in core temperature.en_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.urlhttps://link.springer.com/article/10.1007%2Fs12192-016-0726-0en_US
dc.rightsGreen - can archive pre-print and post-print or publisher's version/PDF
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjecthyperthermiaen_US
dc.subjectcore temperatureen_US
dc.subjectheat acclimationen_US
dc.subjectheat shock proteinsen_US
dc.subjectthermotoleranceen_US
dc.subjectSubject Categories::C600 Sports Scienceen_US
dc.titleHsp72 and Hsp90α mRNA transcription is characterised by large, sustained changes in core temperature during heat acclimationen_US
dc.typeArticleen_US
dc.contributor.departmentBrunel Universityen_US
dc.contributor.departmentUniversity of Brightonen_US
dc.contributor.departmentUniversity of Bedfordshireen_US
dc.contributor.departmentQatar Orthopaedic and Sports Medicine Hospitalen_US
dc.contributor.departmentLoughborough Universityen_US
dc.identifier.journalCell Stress and Chaperonesen_US
dc.identifier.pmcidPMC5083671
dc.date.updated2021-08-13T11:50:58Z
dc.description.notefull text from Uni of Brighton repository


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